{"id":72994,"date":"2026-05-02T06:50:18","date_gmt":"2026-05-02T04:50:18","guid":{"rendered":"https:\/\/chestxray.co.za\/?post_type=product&#038;p=72994"},"modified":"2026-05-02T06:50:25","modified_gmt":"2026-05-02T04:50:25","slug":"m-fc-agar-500g","status":"publish","type":"product","link":"https:\/\/chestxray.co.za\/?product=m-fc-agar-500g","title":{"rendered":"M-FC Agar 500g"},"content":{"rendered":"<p>M-FC Agar 500g<\/p>\n<p>m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.<\/p>\n<p>Many standard method membrane filtration procedures recommend m-FC media for testing water.<br \/> The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 \ufffd\ufffd 0.5\ufffd\ufffdC in the fecal coliform procedure and other tests.<br \/> The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods.<br \/> The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.<br \/> Specifications<br \/> Brand Neogen\ufffd\ufffd Culture Media<br \/> Platform Dehydrated Culture Media<br \/> Mass 5 kg<br \/> Formula<br \/> Formula Liter<br \/> Enzymatic Digest of Casein 10.0 g\/L<br \/> Enzymatic Digest of Animal Tissue 5.0 g\/L<br \/> Yeast Extract 3.0 g\/L<br \/> Sodium Chloride 5.0 g\/L<br \/> Lactose 12.5 g\/L<br \/> Bile Salts 1.5 g\/L<br \/> Aniline Blue 0.1 g\/L<br \/> Agar 15.0 g\/L<br \/> Grams per Liter 52.00<br \/> Liters per 500 g 10.00<br \/> Preparation<br \/> Suspend 52 grams of the medium in one liter of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.<br \/> If necessary, adjust pH to 7.4 with 1N HCl.<br \/> Heat with frequent agitation and boil for one minute to completely dissolve the medium.<br \/> Cool to 45-50\ufffd\ufffdC and pour plates.<br \/> DO NOT AUTOCLAVE.<br \/> Previous Item Number Product was previously known as Acumedia Product: 7397 m FC Agar<br \/> Package Weight 14.00 lb<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>mFC Agar is used <b>for the detection and enumeration of faecal coliforms using membrane filter technique<\/b>.<\/p>\n<p>This medium is recommended by APHA. Store prepared media below 8\ufffd\ufffdC, protected from direct light.<\/p>\n<p>M-FC Agar 500g<\/p>\n","protected":false},"excerpt":{"rendered":"<p>M-FC Agar 500g<\/p>\n<p>m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.<\/p>\n<p>Many standard method membrane filtration procedures recommend m-FC media for testing water.<br \/> The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 \ufffd\ufffd 0.5\ufffd\ufffdC in the fecal coliform procedure and other tests.<br \/> The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods.<br \/> The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.<br \/> Specifications<br \/> Brand Neogen\ufffd\ufffd Culture Media<br \/> Platform Dehydrated Culture Media<br \/> Mass 5 kg<br \/> Formula<br \/> Formula Liter<br \/> Enzymatic Digest of Casein 10.0 g\/L<br \/> Enzymatic Digest of Animal Tissue 5.0 g\/L<br \/> Yeast Extract 3.0 g\/L<br \/> Sodium Chloride 5.0 g\/L<br \/> Lactose 12.5 g\/L<br \/> Bile Salts 1.5 g\/L<br \/> Aniline Blue 0.1 g\/L<br \/> Agar 15.0 g\/L<br \/> Grams per Liter 52.00<br \/> Liters per 500 g 10.00<br \/> Preparation<br \/> Suspend 52 grams of the medium in one liter of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.<br \/> If necessary, adjust pH to 7.4 with 1N HCl.<br \/> Heat with frequent agitation and boil for one minute to completely dissolve the medium.<br \/> Cool to 45-50\ufffd\ufffdC and pour plates.<br \/> DO NOT AUTOCLAVE.<br \/> Previous Item Number Product was previously known as Acumedia Product: 7397 m FC Agar<br \/> Package Weight 14.00 lb<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>mFC Agar is used <b>for the detection and enumeration of faecal coliforms using membrane filter technique<\/b>.<\/p>\n<p>This medium is recommended by APHA. Store prepared media below 8\ufffd\ufffdC, protected from direct light.<\/p>\n<p>M-FC Agar 500g<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"content-type":""},"product_brand":[],"product_cat":[4114],"product_tag":[24179],"class_list":{"0":"post-72994","1":"product","2":"type-product","3":"status-publish","5":"product_cat-laboratory-chemicals","6":"product_tag-m-fc-agar-500g","8":"first","9":"instock","10":"shipping-taxable","11":"purchasable","12":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/product\/72994","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/product"}],"about":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/types\/product"}],"wp:attachment":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=72994"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_brand&post=72994"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_cat&post=72994"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_tag&post=72994"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}