{"id":48413,"date":"2026-04-06T14:26:25","date_gmt":"2026-04-06T12:26:25","guid":{"rendered":"https:\/\/chestxray.co.za\/?post_type=product&p=48413"},"modified":"2026-05-03T08:11:05","modified_gmt":"2026-05-03T06:11:05","slug":"methanol-99-9-for-hplc-gradient-grade-2-5l","status":"publish","type":"product","link":"https:\/\/chestxray.co.za\/?product=methanol-99-9-for-hplc-gradient-grade-2-5l","title":{"rendered":"Methanol 99.9% for HPLC Gradient Grade 2.5L"},"content":{"rendered":"

Methanol 99.9% HPLC<\/strong> (CH\u2083OH,) is one of the most commonly used organic modifiers in reversed-phase chromatography. Although the solvent offers certain advantages and disadvantages, one of Methanol\u2019s key strengths, from a chromatographic perspective, is that it offers substantially different selectivity, and as such, are valuable for method development purposes. <\/p>\n

There are a range of organic solvents that are suitable for use as the organic modifier in reversed-phase liquid chromatography; in practice though, only a few are routinely used. When selecting an organic solvent, properties such as miscibility with water, polarity, UV cut-off, viscosity and safety are important to consider<\/p>\n

Over time, methanol has become one of the organic modifiers of choice for numerous reversed-phase applications. As a solvent it is fully miscible with water and is compatible with common mobile phase additives as well as buffers (care should be taken when using buffer salts at high organic compositions). <\/p>\n

Although Methanol has advantages and disadvantages that should be taken into consideration, some of its key benefits are that it is affordable and less toxic than other organic modifiers. We offer Methanol HPLC in 2,5l bottles; purchase yours online today!<\/p>\n

Methanol 99.9% HPLC \u2013 Identification of Product<\/strong>
Chemical Code: HPLC-M1
Chemical Name: Methanol
Chemical Grade: HPLC
Chemical Formula: CH\u2084O
Chemical Weight: 32,04 g\/mol
CAS No: 67-56-1
Chemical Synonyms: Methanol HPLC<\/p>\n

Methanol 99.9% HPLC \u2013 Hazards Identification<\/strong>
REACH No: 01-2119433307-44-XXXX
Signal Word: Danger
Supplemental Hazard Information:
Additional Hazard Information: No Data Available<\/p>\n

Methanol 99.9% HPLC \u2013 Hazards statements<\/strong>
H225 \u2013 Highly flammable liquid and vapour.H301 \u2013 Toxic if swallowed.
H311 \u2013 Toxic in contact with skin.
H331 \u2013 Toxic if inhaled.
H370 \u2013 Causes damage to organs.<\/p>\n

Methanol 99.9% HPLC \u2013 Precautionary statements<\/strong>
P210 \u2013 Keep away from heat\/sparks\/open flames\/hot surfaces. No smoking.
P260 \u2013 Do not breathe dust\/ fume\/ gas\/ mist\/ vapours\/ spray.
P280 \u2013 Wear protective gloves\/ protective clothing.
P301 + P310 \u2013 IF SWALLOWED: Immediately call a POISON CENTER or doctor\/ physician.
P311 \u2013 Call a POISON CENTER or doctor\/ physician.<\/p>\n

Methanol 99.9% HPLC \u2013 Composition of Chemical<\/strong>
Chemical Formula: CH\u2084O
EC No 1272\/2008: 01-2119433307-44-XXXX<\/p>\n

Methanol 99.9% HPLC \u2013 First Aid Measures<\/strong>
General Advice: Consult a physician. Show this safety data sheet to the doctor in attendance.
If: Inhaled: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
If: Skin Contact: Wash off with soap and plenty of water. Take victim immediately to hospital. Consult a physician.
If: Eye Contac: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If: Swallowed: Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Important Symptoms: Dizziness, Drowsiness, Metabolic acidosis, Blurred vision, Seizures, Coma, Blindness, Death.
Immediate Medical Attention: No Data Available<\/p>\n

Methanol 99.9% HPLC \u2013 Firefighting Measures<\/strong>
Extinguishing Media: Use Water spray, Alcohol-resistant foam, Dry chemical or Carbon Dioxide.
Hazards Arising: Carbon Oxides.
Advice for Firefighters: Wear self-contained breathing apparatus for firefighting if necessary.
Info for Firefighting: Use water spray to cool unopened containers.<\/p>\n

Accidental Release Measures<\/strong>
Personal Precautions: Wear respiratory protection. Avoid breathing vapours, mist or gas.
Ensure adequate ventilation.
Remove all sources of ignition.
Evacuate personnel to safe areas.
Beware of vapours accumulating to form explosive concentrations.
Vapours can accumulate in low areas.
Enviromental Precautions: Prevent further leakage or spillage if safe to do so.
Do not let product enter drains.
Method for Containment: Contain spillage, and then collect with an electrically protected vacuum cleaner or by wet-brushing and place in container for disposal according to local regulations.<\/p>\n

Handling and Storage<\/strong>
Personal Precautions: Avoid contact with skin and eyes.
Avoid inhalation of vapour or mist.
Keep away from sources of ignition \u2013 No smoking.
Take measures to prevent the build up of electrostatic charge.
Enviromental Precautions: Keep container tightly closed in a dry and well-ventilated place.
Containers which are opened must be carefully resealed and kept upright to prevent leakage.
Keep in a cool place.
Exposure Controls | Personal Protection
Derived No Effect Level (DNEL)
Workers | Application Area | Exposure Routes | Health Effect | Value
Workers \u2013 Skin contact \u2013 Long-term systemic effects \u2013 40mg\/kg BW\/d.
Workers \u2013 Skin contact \u2013 Acute systemic effects \u2013 40mg\/kg BW\/d.
Workers \u2013 Inhalation \u2013 Acute systemic effects \u2013 260 mg\/m3.
Workers \u2013 Inhalation \u2013 Acute local effects \u2013 260 mg\/m3.
Workers \u2013 Inhalation \u2013 Long-term systemic effects \u2013 260 mg\/m3.
Workers \u2013 Inhalation \u2013 Long-term local effects \u2013 260 mg\/m3.
Consumers | Application Area | Exposure Routes | Health Effect | Value
Consumers \u2013 Skin contact \u2013 Long-term systemic effects \u2013 8mg\/kg BW\/d.
Consumers \u2013 Ingestion \u2013 Long-term systemic effects \u2013 8mg\/kg BW\/d.
Consumers \u2013 Skin contact \u2013 Acute systemic effects \u2013 8mg\/kg BW\/d.
Consumers \u2013 Ingestion \u2013 Acute systemic effects \u2013 8mg\/kg BW\/d.
Consumers \u2013 Inhalation \u2013 Acute systemic effects 50 mg\/m3.
Consumers \u2013 Inhalation \u2013 Acute local effects 50 mg\/m3.
Consumers \u2013 Inhalation \u2013 Long-term systemic effects \u2013 50 mg\/m3.
Consumers \u2013 Inhalation \u2013 Long-term local effects \u2013 50 mg\/m3.
Predicted No Effect Concentration (PNEC)
Soil \u2013 23,5 mg\/kgMarine water \u2013 15,4 mg\/l
Fresh water \u2013 154 mg\/l
Fresh water sediment \u2013 570,4 mg\/kg
Onsite sewage treatment plant \u2013 100 mg\/kg
Engineering Controls: Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
Eye\/Face Protection: Face shield and safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).
Skin Protection: Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching gloves outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications of EU Directive 89\/686\/EEC and the standard EN 374 derived from it.
Full contact \u2013
Material: Butyl-rubber. Minimum layer thickness: 0,3 mm.
Break through time: 480 min. Material tested: Butoject\u00ae.
Splash contact \u2013
Material: Nitrile rubber. Minimum layer thickness: 0,4 mm.
Break through time: 31 min. Material tested: Camatril\u00ae.
Data source: KCL GmbH, D-36124. Test method: EN374.
If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by an industrial hygienist and safety officer familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval for any specific use scenario.
Body Protection: Complete suit protecting against chemicals. Flame retardant anti-static protective clothing. The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory Protection: Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multipurpose combination (US) or type AXBEK (EN 14387) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).<\/p>\n

Physical and Chemical Properties<\/strong>
Appearance: Clear, Colourless, mobile liquid
Odour: Pungent
Odour Threshold: No Data Available
pH: No Data Available
Melting Point: Melting Point\/Range: -98 \u00b0C
Boiling Point: 64,7 \u00b0CFlash Point: 9,7 \u00b0C (Closed Cup).
Evaporation: No Data Available
Flammability: No Data Available
Upper\/Lower Flammability or Explosive Limits: Upper explosion limit: 36 %(V,
Lower explosion limit: 6 %(V).
Vapour pressure: 130,3 hPa at 20,0 \u00b0C,
546,6 hPa at 50,0 \u00b0C,
169,27 hPa at 25,0 \u00b0C.
Vapour density: 1,11
Relative density: 0,791 g\/mL at 25 \u00b0C
Water solubility: Completely Miscible
Partition Coefficien: Log Pow: -0,77
Auto-ignition Temperature: 455,0 \u00b0C at 1.013 hPa
Decomposition Temperature: No Data Available
Viscosity: No Data Available
Explosive properties: No Data Available
Oxidizing properties: The substance or mixture is not classified as oxidizing.
Other Safety Info: Minimum ignition energy: 0,14 mJ.
Conductivity: < 1 ?S\/cm,
Relative vapour density: 1,11.
Stability and Reactivity
Reactivity: No Data Available
Chemical Stability: Stable under recommended storage conditions.
Possibility of hazardous reactions: No Data Available
Conditions to Avoid: Heat, flames and sparks, Extremes of temperature and direct sunlight.
Incompatible Materials: Acid Chlorides, Acid Anhydrides, Oxidizing agents, Alkali metals, Reducing agents, Acids.
Hazardous Decomposition Products: No Data Available<\/p>\n

Toxicological Information<\/strong>
Acute Toxicity: LDLO Oral \u2013 Human \u2013 143 mg\/kg
Remarks: Lungs, Thorax, or Respiration: Dyspnea. Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhoea.
LD50 Oral \u2013 Rat \u2013 1.187 \u2013 2.769 mg\/kg
LC50 Inhalation \u2013 Rat \u2013 4 h \u2013 128,2 mg\/l
LC50 Inhalation \u2013 Rat \u2013 6 h \u2013 87,6 mg\/lLD50 Dermal \u2013 Rabbit \u2013 17.100 mg\/kg
Skin Corrosion\/Irritation: Rabbit
Result: No skin irritation
Serious Eye damage | Eye Irritation: Rabbit
Result: No eye irritation
Cell Mutagenicity: Ames test \u2013 S. typhimurium \u2013 Result: negative.
In vitro assay \u2013 Fibroblast \u2013 Result: negative \u2013 Mutation in mammalian somatic cells.
Mutagenicity (in vivo mammalian bone-marrow cytogenetic test, chromosomal analysis) \u2013 Mouse \u2013 male and female \u2013 Result: negative
Carcinogenicity: IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity: Damage to fetus not classifiable
Fertility classification not possible from current data.
Specific Target Organ Toxicity \u2013 Single Exposure: Causes damage to organs.
Specific Target Organ Toxicity \u2013 Repeated Exposure: The substance or mixture is not classified as specific target organ toxicant, repeated exposure.
Aspiration Hazard: No aspiration toxicity classification.<\/p>\n

Ecological Information<\/strong>
Ecological Toxicity: Toxicity to fish:
Mortality LC50 \u2013 Lepomis macrochirus (Bluegill) \u2013 15.400,0 mg\/l \u2013 96 h
NOEC \u2013 Oryzias latipes \u2013 7.900 mg\/l \u2013 200 h.
Toxicity to daphnia and other aquatic invertebrates:
EC50 \u2013 Daphnia magna (Water flea) \u2013 > 10.000,00 mg\/l \u2013 48 h.
Toxicity to algae:
Growth inhibition EC50 \u2013 Scenedesmus capricornutum (fresh water algae) \u2013 22.000,0 mg\/l \u2013 96 h.
Ecological Persistence and degradability: Biodegradability:
Aerobic \u2013 Exposure time 5 d \u2013 Result: 72 % \u2013 rapidly biodegradable.
Biochemical Oxygen Demand \u2013 (BOD) \u2013 600 \u2013 1.120 mg\/g.
Chemical Oxygen Demand \u2013 (COD) \u2013 1.420 mg\/g.
Theoretical Oxygen Demand \u2013 1.500 mg\/g
Bioaccumulative Potential: Bio-accumulation: Cyprinus carpio (Carp) \u2013 72 d at 20 \u00b0C \u2013 5 mg\/l.
Bio-concentration factor (BCF): 1,0Mobility in Soil: Will not adsorb on soil.
Results of PBT and vPvB Assessment: PBT\/vPvB assessment not available as chemical safety assessment not required\/not conducted.
Other Adverse Effect: Avoid release to the environment.
Stability in water \u2013 at 19 \u00b0C83 \u2013 91 % \u2013 72 h. Remarks: Hydrolyses on contact with water. Hydrolyses readily.<\/p>\n

Disposal Considerations<\/strong>
Waste Treatment Methods: Product
Burn in a chemical incinerator equipped with an afterburner and scrubber but exert extra care in igniting as this material is highly flammable. Offer surplus and non-recyclable solutions to a licensed disposal company.
Contaminated packaging \u2013 Dispose of as unused product.<\/p>\n

Transport Information<\/strong>
UN Number: ADR\/RID: 1230
IMDG: 1230
IATA: 1230
UN Shipping Hazard: ADR\/RID: METHANOL
IMDG: METHANOL
IATA: Methanol
Transport Hazard Class: ADR\/RID: 3 (6.1)
IMDG: 3 (6.1)
IATA: 3 (6.1)
Packaging Group: ADR\/RID: II
IMDG: II
IATA: II
Enviromental Hazards: ADR\/RID: no
IMDG Marine pollutant: no
IATA: no
Special Precautions: No Data Available<\/p>\n

Regulatory Information<\/strong>
Safety, Health and environmental regulations: This safety datasheet complies with the requirements of Regulation (EC) No. 1907\/2006.
International Chemical Weapons Convention (CWC) Schedules of Toxic Chemicals and Precursors: Neither banned nor restricted.
REACH \u2013 Restrictions on the manufacture, placing on the market and use of certain dangerous substances, preparations and articles (Annex XVII):
Neither banned nor restricted.
Regulation (EC) No 649\/2012 of the European Parliament and the Council concerning the export and import of dangerous chemicals:
Neither banned nor restricted.
REACH \u2013 Candidate List of Substances of Very High Concern for Authorization (Article 59): This product does not contain substances of very high concern (Regulation (EC) No. 1907\/2006 (REACH), Article 57). REACH \u2013 List of substances subject to authorization (Annex XIV): Neither banned nor restricted.
Regulation (EC) No 1005\/2009 on substances that deplete the ozone layer: Neither banned nor restricted.
Regulation (EC) No 850\/2004 on persistent organic pollutants: Neither banned nor restricted.
Chemical Safety Assessment: A Chemical Safety Assessment has been carried out for this substance.
Additional Info: RTECS: PC1400000.
Effects due to ingestion may include: Headache, Dizziness, Drowsiness, Metabolic acidosis, Coma, Seizures.
Methyl alcohol may be fatal or cause blindness if swallowed.<\/p>\n

Disclaimer<\/strong>
The information stated above is considered to be correct, but does not claim to be inclusive and shall only be used as a guideline. The information provided by this document is confirmed by our continuous updating of knowledge and adheres to the products appropriate safety precautions. It does not represent any guarantee of the product\u2019s
properties. RLS Chemicals and its Associates shall not be held accountable for any damage\u2019s consequent of handling the above product<\/p>\n

Add to Wishlist<\/p>\n

Add to Wishlist<\/p>\n","protected":false},"excerpt":{"rendered":"

Chemical Formula:<\/strong> CH3COCH3
Molar Mass:<\/strong> 58.08g\/mol
Melting Point:<\/strong> -97.8\u00b0C
Boiling Point:<\/strong> 64.4\u00b0C
Ignition Point:<\/strong> 455\u00b0C
Appearance:<\/strong> Colourless liquid
Density @20\u00b0C (g\/ml)<\/strong>: 0.7915g\/ml<\/p>\n

Methanol 99.9% (Gradient Grade), a premium solvent that has been specially formulated for outstanding performance in HPLC gradient applications. With its high purity level of 99.9%, this solvent is designed to deliver superior results in chromatographic analyses.
Methanol 99.9% (Gradient Grade) boasts low UV absorbance, minimal water content, and consistent quality, all of which are crucial factors for reliable separation, accurate calibration, and reproducible outcomes in HPLC procedures. By utilizing this solvent, you can enhance the efficiency and precision of your experiments, thereby achieving more consistent and dependable results.<\/p>\n","protected":false},"featured_media":53675,"template":"","meta":[],"product_brand":[],"product_cat":[24937],"product_tag":[10607],"class_list":["post-48413","product","type-product","status-publish","has-post-thumbnail","product_cat-other-miscellaneous-other-disposable-consumables-items","product_tag-methanol-99-9-for-hplc-gradient-grade-2-5l","first","instock","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/product\/48413","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/product"}],"about":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=\/wp\/v2\/media\/53675"}],"wp:attachment":[{"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=48413"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_brand&post=48413"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_cat&post=48413"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/chestxray.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_tag&post=48413"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}